Influence of auxin and cytokinine on in vitro multiplication of Ficus anastasia

The role of cytokinine and auxin has been found to be effective in shoot multiplication of Ficus anastasia. When the multiplication medium contained 8 mgl-1 of BA with 1 mgl-1 of IBA, a mean of 20 lateral branches per stem was induced which was the best combination recorded. Even though IAAparticularly combined with 2ip or kinetin, it had weak shoot multiplication influence resulting in only two lateral shoots which increased to 7 lateral shoots per stem when combined with BA. The plant height was negatively affected by the increase of cytokinin concentration. However, the control plantwas more in length than any of the cytokinin treatments among combination with IBA or IAA

RAPD-based detection of genomic instability in cucumber plants derived from somatic embryogenesis

Random amplified polymorphic DNA (RAPD) markers were used to evaluate genetic stability of regenerants of cucumber plants obtained through somatic embryogenesis. Somatic embryo plants and plants of F1 hybrids, from which they were derived, were compared during weaning, early growth, flowering, fruiting and at maturity. No differences in phenotype were observed, by simple observation. Banding patterns were scored for the presence (+) or absence (-) of a DNA band, there were no visually detectable differences between the somatic embryo derived plants compared to their F1 parents in the Random Amplified Polymorphic DNA (RAPD) test using five primers OP-C10, OP-G14, OP-H05, OP-Y03 and OP-AT01. The results indicate the usefulness of RAPD markers to detect genetic instability in cucumber primary regenerant plants derived from somatic embryogenesis, and as a certification tool for monitoring genetic stability during the generation process